wm 779 Search Results


wm 779  (ATCC)
94
ATCC wm 779
Wm 779, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wm 779/product/ATCC
Average 94 stars, based on 1 article reviews
wm 779 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
serotype c c gattii isolates  (ATCC)
94
ATCC serotype c c gattii isolates
A . Molecular type analysis. Determination of the major molecular types via electrophoretic separation of URA5 gene restriction patterns after double digestion with Hha I and Sau 96I obtained from isolates 106.97 (VGIV (2)), ATCC 24066 (VGIII (3)), WM179 (VGI reference strain (5)), WM178 (VGII reference strain (6)), WM175 (VGIII reference strain (7)) and WM 779 (VGIV reference strain (8)), 1, 4, 9 = 1 Kb extension ladder (Invitrogen, USA). The gel showed in this panel is representative of different experiments performed under the same experimental conditions. Cropping lines are indicated in white and the unedited gel is available as . B . MSP dendrograms grouping mass spectra of C. <t>gattii</t> isolates according to their major molecular type. Isolates used in this analysis other than the three currently studied (bold) were obtained from the in-house MALDI_Biotyper BDAL MSP library at Westmead Hospital, Westmead Millennium Culture Collection at Sydney University – Sydney Medical School .
Serotype C C Gattii Isolates, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/serotype c c gattii isolates/product/ATCC
Average 94 stars, based on 1 article reviews
serotype c c gattii isolates - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
biot-egpwleeeeaygwmdf-nh2  (Biosynth Carbosynth)
N/A
   Buy from Supplier
pwm 12x601 35bplinker plasmid 157790  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
pwm 12x601 45bplinker plasmid 157791  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
h-cggwmed(py)dyvh-oh  (Biosynth Carbosynth)
N/A
   Buy from Supplier

Image Search Results


A . Molecular type analysis. Determination of the major molecular types via electrophoretic separation of URA5 gene restriction patterns after double digestion with Hha I and Sau 96I obtained from isolates 106.97 (VGIV (2)), ATCC 24066 (VGIII (3)), WM179 (VGI reference strain (5)), WM178 (VGII reference strain (6)), WM175 (VGIII reference strain (7)) and WM 779 (VGIV reference strain (8)), 1, 4, 9 = 1 Kb extension ladder (Invitrogen, USA). The gel showed in this panel is representative of different experiments performed under the same experimental conditions. Cropping lines are indicated in white and the unedited gel is available as . B . MSP dendrograms grouping mass spectra of C. gattii isolates according to their major molecular type. Isolates used in this analysis other than the three currently studied (bold) were obtained from the in-house MALDI_Biotyper BDAL MSP library at Westmead Hospital, Westmead Millennium Culture Collection at Sydney University – Sydney Medical School .

Journal: Scientific Reports

Article Title: Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

doi: 10.1038/srep11717

Figure Lengend Snippet: A . Molecular type analysis. Determination of the major molecular types via electrophoretic separation of URA5 gene restriction patterns after double digestion with Hha I and Sau 96I obtained from isolates 106.97 (VGIV (2)), ATCC 24066 (VGIII (3)), WM179 (VGI reference strain (5)), WM178 (VGII reference strain (6)), WM175 (VGIII reference strain (7)) and WM 779 (VGIV reference strain (8)), 1, 4, 9 = 1 Kb extension ladder (Invitrogen, USA). The gel showed in this panel is representative of different experiments performed under the same experimental conditions. Cropping lines are indicated in white and the unedited gel is available as . B . MSP dendrograms grouping mass spectra of C. gattii isolates according to their major molecular type. Isolates used in this analysis other than the three currently studied (bold) were obtained from the in-house MALDI_Biotyper BDAL MSP library at Westmead Hospital, Westmead Millennium Culture Collection at Sydney University – Sydney Medical School .

Article Snippet: In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779).

Techniques:

MALDI-TOF MS identification (ID) of the C. gattii isolates analyzed in this study.

Journal: Scientific Reports

Article Title: Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

doi: 10.1038/srep11717

Figure Lengend Snippet: MALDI-TOF MS identification (ID) of the C. gattii isolates analyzed in this study.

Article Snippet: In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779).

Techniques:

A . Microscopic analysis of fungal cells after sequential staining with GXM mAbs (green), calcofluor White (blue) and counter-staining with India ink. Each mAb used for GXM detection is indicated on the left. Scale bar: 5 μm. B . Flow cytometry analysis of the reactivity of GXM-binding mAbs with the serotype C C. gattii isolates. Insets denotes the fluorescence of fungal cells in systems where primary antibodies were not used.

Journal: Scientific Reports

Article Title: Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

doi: 10.1038/srep11717

Figure Lengend Snippet: A . Microscopic analysis of fungal cells after sequential staining with GXM mAbs (green), calcofluor White (blue) and counter-staining with India ink. Each mAb used for GXM detection is indicated on the left. Scale bar: 5 μm. B . Flow cytometry analysis of the reactivity of GXM-binding mAbs with the serotype C C. gattii isolates. Insets denotes the fluorescence of fungal cells in systems where primary antibodies were not used.

Article Snippet: In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779).

Techniques: Staining, Flow Cytometry, Binding Assay, Fluorescence

A . India ink counter-staining (left panels) and corresponding capsular dimensions (right panels) of C. gattii after growth in Sabouraud or minimal media. Capsular dimensions of the ATCC 24066 isolate were significantly smaller than those observed for 106.97 and WM 779 isolates (*** p = <0.0005). Scale bar: 5 μm. B . Expression of CAP genes under the conditions used for determination of capsular dimensions. CAP59 and CAP60 had their expression significantly altered in the ATCC 24066 isolate (*** p = <0.0001; ** p = <0.005).

Journal: Scientific Reports

Article Title: Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

doi: 10.1038/srep11717

Figure Lengend Snippet: A . India ink counter-staining (left panels) and corresponding capsular dimensions (right panels) of C. gattii after growth in Sabouraud or minimal media. Capsular dimensions of the ATCC 24066 isolate were significantly smaller than those observed for 106.97 and WM 779 isolates (*** p = <0.0005). Scale bar: 5 μm. B . Expression of CAP genes under the conditions used for determination of capsular dimensions. CAP59 and CAP60 had their expression significantly altered in the ATCC 24066 isolate (*** p = <0.0001; ** p = <0.005).

Article Snippet: In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779).

Techniques: Staining, Expressing

A . In vitro and in vivo detection of chitooligomers using TRITC-WGA. The typical polarized pattern of chitooligomer detection was observed in vitro . In vivo analysis revealed annular patterns of oligomer detection in isolates 106.97 and WM 779 and negative staining for the ATCC 24066 isolate. Scale bar, 5 μm. B . Pulmonary chitinase activity after infection with C. gattii . From day 14 to 21 post infection, chitinase activity was significantly higher when mice was infected with isolates 106.97 or WM 779, in comparison to the ATCC 24066 isolate (P < 0.05).

Journal: Scientific Reports

Article Title: Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

doi: 10.1038/srep11717

Figure Lengend Snippet: A . In vitro and in vivo detection of chitooligomers using TRITC-WGA. The typical polarized pattern of chitooligomer detection was observed in vitro . In vivo analysis revealed annular patterns of oligomer detection in isolates 106.97 and WM 779 and negative staining for the ATCC 24066 isolate. Scale bar, 5 μm. B . Pulmonary chitinase activity after infection with C. gattii . From day 14 to 21 post infection, chitinase activity was significantly higher when mice was infected with isolates 106.97 or WM 779, in comparison to the ATCC 24066 isolate (P < 0.05).

Article Snippet: In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779).

Techniques: In Vitro, In Vivo, Negative Staining, Activity Assay, Infection, Comparison

( A ) and fungal burden ( B,C ) after infection of mice with serotype C isolates of C. gattii . A. Mice infected with the ATCC 24066 isolate lived longer than those infected with isolates 106.97 and WM 779 ( P = 0.0003). B. Pulmonary burden after infection with isolate ATCC 24066 was always smaller (P<0.05) in comparison with systems where animals were inoculated with isolates 106.97 or WM 779. C. Brain colonization after infection of mice with isolates 106.96 and WM 779. Isolate WM 779 took longer to infect the brain of mice, but fungal burden 21 days post-infection was significantly higher for this isolate. Negative results were obtained when fungal colonization was analyzed after infection with isolate ATCC 24066. Isolate 106.97, blue bars; isolate WM 779, orange bars.

Journal: Scientific Reports

Article Title: Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates

doi: 10.1038/srep11717

Figure Lengend Snippet: ( A ) and fungal burden ( B,C ) after infection of mice with serotype C isolates of C. gattii . A. Mice infected with the ATCC 24066 isolate lived longer than those infected with isolates 106.97 and WM 779 ( P = 0.0003). B. Pulmonary burden after infection with isolate ATCC 24066 was always smaller (P<0.05) in comparison with systems where animals were inoculated with isolates 106.97 or WM 779. C. Brain colonization after infection of mice with isolates 106.96 and WM 779. Isolate WM 779 took longer to infect the brain of mice, but fungal burden 21 days post-infection was significantly higher for this isolate. Negative results were obtained when fungal colonization was analyzed after infection with isolate ATCC 24066. Isolate 106.97, blue bars; isolate WM 779, orange bars.

Article Snippet: In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779).

Techniques: Infection, Comparison